Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
1 |
1.6% |
| Arg[R] |
2 |
3.2% |
| Asn[N] |
3 |
4.8% |
| Asp[D] |
0 |
0.0% |
| Cys[C] |
1 |
1.6% |
| Gln[Q] |
2 |
3.2% |
| Glu[E] |
0 |
0.0% |
| Gly[G] |
2 |
3.2% |
| His[H] |
3 |
4.8% |
| Ile[I] |
3 |
4.8% |
| Leu[L] |
11 |
17.7% |
| Lys[K] |
10 |
16.1% |
| Met[M] |
3 |
4.8% |
| Phe[F] |
3 |
4.8% |
| Pro[P] |
4 |
6.5% |
| Ser[S] |
3 |
4.8% |
| Thr[T] |
5 |
8.1% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
2 |
3.2% |
| Val[V] |
4 |
6.5% |
| Total Length |
62 |
|
| Instability index (II) |
61.08 |
| This classifies the protein as unstable |
|
| Carbon |
335 |
| Hydrogen |
557 |
| Nitrogen |
89 |
| Oxygen |
78 |
| Sulfur |
4 |
| Total number of atoms |
1063 |
| Formula = C335H557N89O78S4 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
2980 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
2980 M-1 cm-1 |
| assuming all Cys residues are reduced |
2980 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
2980 M-1 cm-1 |
|
|
| Codon Bias |
-0.265 |
| Codon Adaptation Index |
0.050 |
| Frequency of Optimal Codons |
0.237 |
| Hydropathicity of Protein |
-0.005 |
| Aromaticity Score |
0.081 |
|
