YHR073W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 3 5.2%
Arg[R] 6 10.3%
Asn[N] 2 3.4%
Asp[D] 3 5.2%
Cys[C] 1 1.7%
Gln[Q] 1 1.7%
Glu[E] 0 0.0%
Gly[G] 2 3.4%
His[H] 2 3.4%
Ile[I] 4 6.9%
Leu[L] 6 10.3%
Lys[K] 5 8.6%
Met[M] 1 1.7%
Phe[F] 1 1.7%
Pro[P] 4 6.9%
Ser[S] 7 12.1%
Thr[T] 2 3.4%
Trp[W] 2 3.4%
Tyr[Y] 3 5.2%
Val[V] 3 5.2%
Total Length 58

Instability index
Instability index (II) 59.60
This classifies the protein as unstable

Atomic composition
Carbon 306
Hydrogen 488
Nitrogen 90
Oxygen 80
Sulfur 2
Total number of atoms 966
Formula = C306H488N90O80S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 15470 M-1 cm-1
assuming NO Cys residues appear as half cystines 15470 M-1 cm-1
assuming all Cys residues are reduced 15470 M-1 cm-1
assuming all pairs of Cys residues form cystines 15470 M-1 cm-1

Aliphatic index
Aliphatic index 87.41

Coding Region Translation Calculations
Codon Bias -0.080
Codon Adaptation Index 0.065
Frequency of Optimal Codons 0.327
Hydropathicity of Protein -0.479
Aromaticity Score 0.103