YBR196C-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 3 8.8%
Arg[R] 2 5.9%
Asn[N] 0 0.0%
Asp[D] 0 0.0%
Cys[C] 0 0.0%
Gln[Q] 0 0.0%
Glu[E] 2 5.9%
Gly[G] 2 5.9%
His[H] 0 0.0%
Ile[I] 2 5.9%
Leu[L] 6 17.6%
Lys[K] 5 14.7%
Met[M] 1 2.9%
Phe[F] 1 2.9%
Pro[P] 0 0.0%
Ser[S] 2 5.9%
Thr[T] 1 2.9%
Trp[W] 1 2.9%
Tyr[Y] 2 5.9%
Val[V] 4 11.8%
Total Length 34

Instability index
Instability index (II) 22.59
This classifies the protein as stable

Atomic composition
Carbon 186
Hydrogen 308
Nitrogen 46
Oxygen 44
Sulfur 1
Total number of atoms 585
Formula = C186H308N46O44S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 8480 M-1 cm-1

Aliphatic index
Aliphatic index 134.71

Coding Region Translation Calculations
Codon Bias -0.004
Codon Adaptation Index 0.087
Frequency of Optimal Codons 0.375
Hydropathicity of Protein 0.488
Aromaticity Score 0.118