YJL136W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 1 3.7%
Arg[R] 1 3.7%
Asn[N] 0 0.0%
Asp[D] 1 3.7%
Cys[C] 1 3.7%
Gln[Q] 0 0.0%
Glu[E] 1 3.7%
Gly[G] 0 0.0%
His[H] 1 3.7%
Ile[I] 2 7.4%
Leu[L] 5 18.5%
Lys[K] 2 7.4%
Met[M] 3 11.1%
Phe[F] 0 0.0%
Pro[P] 1 3.7%
Ser[S] 4 14.8%
Thr[T] 1 3.7%
Trp[W] 0 0.0%
Tyr[Y] 2 7.4%
Val[V] 1 3.7%
Total Length 27

Instability index
Instability index (II) 63.89
This classifies the protein as unstable

Atomic composition
Carbon 140
Hydrogen 232
Nitrogen 34
Oxygen 39
Sulfur 4
Total number of atoms 449
Formula = C140H232N34O39S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1
assuming NO Cys residues appear as half cystines 2980 M-1 cm-1
assuming all Cys residues are reduced 2980 M-1 cm-1
assuming all pairs of Cys residues form cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 115.56

Coding Region Translation Calculations
Codon Bias -0.146
Codon Adaptation Index 0.068
Frequency of Optimal Codons 0.292
Hydropathicity of Protein 0.430
Aromaticity Score 0.074