Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
9 |
4.5% |
| Arg[R] |
13 |
6.5% |
| Asn[N] |
9 |
4.5% |
| Asp[D] |
5 |
2.5% |
| Cys[C] |
11 |
5.5% |
| Gln[Q] |
1 |
0.5% |
| Glu[E] |
5 |
2.5% |
| Gly[G] |
2 |
1.0% |
| His[H] |
2 |
1.0% |
| Ile[I] |
9 |
4.5% |
| Leu[L] |
31 |
15.6% |
| Lys[K] |
5 |
2.5% |
| Met[M] |
9 |
4.5% |
| Phe[F] |
29 |
14.6% |
| Pro[P] |
6 |
3.0% |
| Ser[S] |
34 |
17.1% |
| Thr[T] |
10 |
5.0% |
| Trp[W] |
2 |
1.0% |
| Tyr[Y] |
1 |
0.5% |
| Val[V] |
6 |
3.0% |
| Total Length |
199 |
|
| Instability index (II) |
56.03 |
| This classifies the protein as unstable |
|
| Carbon |
1049 |
| Hydrogen |
1607 |
| Nitrogen |
259 |
| Oxygen |
275 |
| Sulfur |
20 |
| Total number of atoms |
3210 |
| Formula = C1049H1607N259O275S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
13115 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
12490 M-1 cm-1 |
| assuming all Cys residues are reduced |
12490 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
13115 M-1 cm-1 |
|
|
| Codon Bias |
0.095 |
| Codon Adaptation Index |
0.113 |
| Frequency of Optimal Codons |
0.436 |
| Hydropathicity of Protein |
0.620 |
| Aromaticity Score |
0.161 |
|
