YHR028W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 5 4.7%
Arg[R] 5 4.7%
Asn[N] 5 4.7%
Asp[D] 0 0.0%
Cys[C] 2 1.9%
Gln[Q] 1 0.9%
Glu[E] 5 4.7%
Gly[G] 5 4.7%
His[H] 1 0.9%
Ile[I] 13 12.3%
Leu[L] 10 9.4%
Lys[K] 4 3.8%
Met[M] 5 4.7%
Phe[F] 6 5.7%
Pro[P] 3 2.8%
Ser[S] 19 17.9%
Thr[T] 5 4.7%
Trp[W] 1 0.9%
Tyr[Y] 7 6.6%
Val[V] 4 3.8%
Total Length 106

Instability index
Instability index (II) 64.30
This classifies the protein as unstable

Atomic composition
Carbon 544
Hydrogen 852
Nitrogen 134
Oxygen 154
Sulfur 7
Total number of atoms 1691
Formula = C544H852N134O154S7

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 16055 M-1 cm-1
assuming NO Cys residues appear as half cystines 15930 M-1 cm-1
assuming all Cys residues are reduced 15930 M-1 cm-1
assuming all pairs of Cys residues form cystines 16055 M-1 cm-1

Aliphatic index
Aliphatic index 100.28

Coding Region Translation Calculations
Codon Bias -0.013
Codon Adaptation Index 0.087
Frequency of Optimal Codons 0.400
Hydropathicity of Protein 0.361
Aromaticity Score 0.132