YER006C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 4 3.8%
Arg[R] 4 3.8%
Asn[N] 9 8.6%
Asp[D] 1 1.0%
Cys[C] 0 0.0%
Gln[Q] 1 1.0%
Glu[E] 5 4.8%
Gly[G] 2 1.9%
His[H] 3 2.9%
Ile[I] 12 11.4%
Leu[L] 6 5.7%
Lys[K] 10 9.5%
Met[M] 4 3.8%
Phe[F] 9 8.6%
Pro[P] 6 5.7%
Ser[S] 13 12.4%
Thr[T] 4 3.8%
Trp[W] 1 1.0%
Tyr[Y] 7 6.7%
Val[V] 4 3.8%
Total Length 105

Instability index
Instability index (II) 51.50
This classifies the protein as unstable

Atomic composition
Carbon 576
Hydrogen 878
Nitrogen 144
Oxygen 152
Sulfur 4
Total number of atoms 1754
Formula = C576H878N144O152S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 15930 M-1 cm-1

Aliphatic index
Aliphatic index 81.71

Coding Region Translation Calculations
Codon Bias -0.097
Codon Adaptation Index 0.124
Frequency of Optimal Codons 0.380
Hydropathicity of Protein -0.215
Aromaticity Score 0.162