Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
3 |
4.5% |
| Arg[R] |
2 |
3.0% |
| Asn[N] |
3 |
4.5% |
| Asp[D] |
3 |
4.5% |
| Cys[C] |
14 |
21.2% |
| Gln[Q] |
8 |
12.1% |
| Glu[E] |
0 |
0.0% |
| Gly[G] |
4 |
6.1% |
| His[H] |
1 |
1.5% |
| Ile[I] |
4 |
6.1% |
| Leu[L] |
4 |
6.1% |
| Lys[K] |
0 |
0.0% |
| Met[M] |
3 |
4.5% |
| Phe[F] |
0 |
0.0% |
| Pro[P] |
9 |
13.6% |
| Ser[S] |
2 |
3.0% |
| Thr[T] |
0 |
0.0% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
5 |
7.6% |
| Val[V] |
1 |
1.5% |
| Total Length |
66 |
|
| Instability index (II) |
64.62 |
| This classifies the protein as unstable |
|
| Carbon |
305 |
| Hydrogen |
469 |
| Nitrogen |
85 |
| Oxygen |
91 |
| Sulfur |
17 |
| Total number of atoms |
967 |
| Formula = C305H469N85O91S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
8325 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
7450 M-1 cm-1 |
| assuming all Cys residues are reduced |
7450 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
8325 M-1 cm-1 |
|
|
| Codon Bias |
0.220 |
| Codon Adaptation Index |
0.178 |
| Frequency of Optimal Codons |
0.540 |
| Hydropathicity of Protein |
-0.027 |
| Aromaticity Score |
0.076 |
|
