YPR160C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 5 5.3%
Arg[R] 4 4.3%
Asn[N] 4 4.3%
Asp[D] 2 2.1%
Cys[C] 6 6.4%
Gln[Q] 1 1.1%
Glu[E] 3 3.2%
Gly[G] 4 4.3%
His[H] 1 1.1%
Ile[I] 9 9.6%
Leu[L] 10 10.6%
Lys[K] 8 8.5%
Met[M] 3 3.2%
Phe[F] 3 3.2%
Pro[P] 4 4.3%
Ser[S] 13 13.8%
Thr[T] 7 7.4%
Trp[W] 2 2.1%
Tyr[Y] 1 1.1%
Val[V] 4 4.3%
Total Length 94

Instability index
Instability index (II) 43.50
This classifies the protein as unstable

Atomic composition
Carbon 457
Hydrogen 753
Nitrogen 123
Oxygen 131
Sulfur 9
Total number of atoms 1473
Formula = C457H753N123O131S9

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 12865 M-1 cm-1
assuming NO Cys residues appear as half cystines 12490 M-1 cm-1
assuming all Cys residues are reduced 12490 M-1 cm-1
assuming all pairs of Cys residues form cystines 12865 M-1 cm-1

Aliphatic index
Aliphatic index 96.49

Coding Region Translation Calculations
Codon Bias -0.036
Codon Adaptation Index 0.096
Frequency of Optimal Codons 0.382
Hydropathicity of Protein 0.209
Aromaticity Score 0.064