YPL135C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 3 5.3%
Arg[R] 1 1.8%
Asn[N] 4 7.0%
Asp[D] 0 0.0%
Cys[C] 2 3.5%
Gln[Q] 0 0.0%
Glu[E] 3 5.3%
Gly[G] 3 5.3%
His[H] 2 3.5%
Ile[I] 3 5.3%
Leu[L] 8 14.0%
Lys[K] 1 1.8%
Met[M] 1 1.8%
Phe[F] 2 3.5%
Pro[P] 8 14.0%
Ser[S] 5 8.8%
Thr[T] 7 12.3%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 4 7.0%
Total Length 57

Instability index
Instability index (II) 43.34
This classifies the protein as unstable

Atomic composition
Carbon 268
Hydrogen 433
Nitrogen 69
Oxygen 80
Sulfur 3
Total number of atoms 853
Formula = C268H433N69O80S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 125 M-1 cm-1
assuming NO Cys residues appear as half cystines 0 M-1 cm-1
assuming all Cys residues are reduced 0 M-1 cm-1
assuming all pairs of Cys residues form cystines 125 M-1 cm-1

Aliphatic index
Aliphatic index 100.88

Coding Region Translation Calculations
Codon Bias -0.005
Codon Adaptation Index 0.067
Frequency of Optimal Codons 0.375
Hydropathicity of Protein 0.288
Aromaticity Score 0.035