YOR161W-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 6 7.0%
Arg[R] 6 7.0%
Asn[N] 4 4.7%
Asp[D] 2 2.3%
Cys[C] 1 1.2%
Gln[Q] 3 3.5%
Glu[E] 3 3.5%
Gly[G] 1 1.2%
His[H] 1 1.2%
Ile[I] 6 7.0%
Leu[L] 5 5.8%
Lys[K] 6 7.0%
Met[M] 7 8.1%
Phe[F] 3 3.5%
Pro[P] 8 9.3%
Ser[S] 4 4.7%
Thr[T] 9 10.5%
Trp[W] 0 0.0%
Tyr[Y] 4 4.7%
Val[V] 7 8.1%
Total Length 86

Instability index
Instability index (II) 49.51
This classifies the protein as unstable

Atomic composition
Carbon 442
Hydrogen 719
Nitrogen 119
Oxygen 121
Sulfur 8
Total number of atoms 1409
Formula = C442H719N119O121S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 5960 M-1 cm-1
assuming NO Cys residues appear as half cystines 5960 M-1 cm-1
assuming all Cys residues are reduced 5960 M-1 cm-1
assuming all pairs of Cys residues form cystines 5960 M-1 cm-1

Aliphatic index
Aliphatic index 80.47

Coding Region Translation Calculations
Codon Bias -0.052
Codon Adaptation Index 0.064
Frequency of Optimal Codons 0.380
Hydropathicity of Protein -0.152
Aromaticity Score 0.081