YML133W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 19 9.9%
Arg[R] 6 3.1%
Asn[N] 1 0.5%
Asp[D] 6 3.1%
Cys[C] 0 0.0%
Gln[Q] 0 0.0%
Glu[E] 2 1.0%
Gly[G] 40 20.9%
His[H] 0 0.0%
Ile[I] 14 7.3%
Leu[L] 7 3.7%
Lys[K] 0 0.0%
Met[M] 2 1.0%
Phe[F] 6 3.1%
Pro[P] 5 2.6%
Ser[S] 46 24.1%
Thr[T] 18 9.4%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 19 9.9%
Total Length 191

Instability index
Instability index (II) 27.77
This classifies the protein as stable

Atomic composition
Carbon 731
Hydrogen 1204
Nitrogen 210
Oxygen 273
Sulfur 2
Total number of atoms 2420
Formula = C731H1204N210O273S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours


Aliphatic index
Aliphatic index 81.68

Coding Region Translation Calculations
Codon Bias 0.284
Codon Adaptation Index 0.152
Frequency of Optimal Codons 0.545
Hydropathicity of Protein 0.483
Aromaticity Score 0.031