YLR286W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 1 2.3%
Arg[R] 3 6.8%
Asn[N] 2 4.5%
Asp[D] 0 0.0%
Cys[C] 3 6.8%
Gln[Q] 3 6.8%
Glu[E] 1 2.3%
Gly[G] 2 4.5%
His[H] 0 0.0%
Ile[I] 4 9.1%
Leu[L] 2 4.5%
Lys[K] 0 0.0%
Met[M] 1 2.3%
Phe[F] 6 13.6%
Pro[P] 1 2.3%
Ser[S] 6 13.6%
Thr[T] 7 15.9%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 2 4.5%
Total Length 44

Instability index
Instability index (II) 47.65
This classifies the protein as unstable

Atomic composition
Carbon 218
Hydrogen 340
Nitrogen 58
Oxygen 65
Sulfur 4
Total number of atoms 685
Formula = C218H340N58O65S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 125 M-1 cm-1
assuming NO Cys residues appear as half cystines 0 M-1 cm-1
assuming all Cys residues are reduced 0 M-1 cm-1
assuming all pairs of Cys residues form cystines 125 M-1 cm-1

Aliphatic index
Aliphatic index 68.64

Coding Region Translation Calculations
Codon Bias 0.184
Codon Adaptation Index 0.164
Frequency of Optimal Codons 0.535
Hydropathicity of Protein 0.350
Aromaticity Score 0.136