YHR214C-E Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 3 3.0%
Arg[R] 5 5.1%
Asn[N] 5 5.1%
Asp[D] 4 4.0%
Cys[C] 5 5.1%
Gln[Q] 5 5.1%
Glu[E] 1 1.0%
Gly[G] 5 5.1%
His[H] 2 2.0%
Ile[I] 8 8.1%
Leu[L] 15 15.2%
Lys[K] 6 6.1%
Met[M] 3 3.0%
Phe[F] 8 8.1%
Pro[P] 2 2.0%
Ser[S] 4 4.0%
Thr[T] 6 6.1%
Trp[W] 2 2.0%
Tyr[Y] 4 4.0%
Val[V] 6 6.1%
Total Length 99

Instability index
Instability index (II) 25.38
This classifies the protein as stable

Atomic composition
Carbon 537
Hydrogen 838
Nitrogen 136
Oxygen 134
Sulfur 8
Total number of atoms 1653
Formula = C537H838N136O134S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 17210 M-1 cm-1
assuming NO Cys residues appear as half cystines 16960 M-1 cm-1
assuming all Cys residues are reduced 16960 M-1 cm-1
assuming all pairs of Cys residues form cystines 17210 M-1 cm-1

Aliphatic index
Aliphatic index 111.21

Coding Region Translation Calculations
Codon Bias -0.081
Codon Adaptation Index 0.107
Frequency of Optimal Codons 0.362
Hydropathicity of Protein 0.402
Aromaticity Score 0.141