YHR214C-D Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 3 3.1%
Arg[R] 1 1.0%
Asn[N] 4 4.1%
Asp[D] 4 4.1%
Cys[C] 3 3.1%
Gln[Q] 1 1.0%
Glu[E] 4 4.1%
Gly[G] 4 4.1%
His[H] 4 4.1%
Ile[I] 11 11.3%
Leu[L] 13 13.4%
Lys[K] 3 3.1%
Met[M] 3 3.1%
Phe[F] 7 7.2%
Pro[P] 2 2.1%
Ser[S] 10 10.3%
Thr[T] 4 4.1%
Trp[W] 2 2.1%
Tyr[Y] 2 2.1%
Val[V] 12 12.4%
Total Length 97

Instability index
Instability index (II) 14.91
This classifies the protein as stable

Atomic composition
Carbon 509
Hydrogen 792
Nitrogen 118
Oxygen 135
Sulfur 6
Total number of atoms 1560
Formula = C509H792N118O135S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 14105 M-1 cm-1
assuming NO Cys residues appear as half cystines 13980 M-1 cm-1
assuming all Cys residues are reduced 13980 M-1 cm-1
assuming all pairs of Cys residues form cystines 14105 M-1 cm-1

Aliphatic index
Aliphatic index 135.46

Coding Region Translation Calculations
Codon Bias -0.043
Codon Adaptation Index 0.103
Frequency of Optimal Codons 0.402
Hydropathicity of Protein 0.959
Aromaticity Score 0.113