YHR054W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 0 0.0%
Arg[R] 5 7.8%
Asn[N] 1 1.6%
Asp[D] 1 1.6%
Cys[C] 4 6.2%
Gln[Q] 2 3.1%
Glu[E] 0 0.0%
Gly[G] 6 9.4%
His[H] 4 6.2%
Ile[I] 8 12.5%
Leu[L] 4 6.2%
Lys[K] 1 1.6%
Met[M] 3 4.7%
Phe[F] 5 7.8%
Pro[P] 1 1.6%
Ser[S] 7 10.9%
Thr[T] 5 7.8%
Trp[W] 1 1.6%
Tyr[Y] 5 7.8%
Val[V] 1 1.6%
Total Length 64

Instability index
Instability index (II) 62.80
This classifies the protein as unstable

Atomic composition
Carbon 341
Hydrogen 512
Nitrogen 92
Oxygen 87
Sulfur 7
Total number of atoms 1039
Formula = C341H512N92O87S7

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 13200 M-1 cm-1
assuming NO Cys residues appear as half cystines 12950 M-1 cm-1
assuming all Cys residues are reduced 12950 M-1 cm-1
assuming all pairs of Cys residues form cystines 13200 M-1 cm-1

Aliphatic index
Aliphatic index 77.66

Coding Region Translation Calculations
Codon Bias 0.046
Codon Adaptation Index 0.128
Frequency of Optimal Codons 0.450
Hydropathicity of Protein 0.178
Aromaticity Score 0.172