YHR022C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 2 6.9%
Arg[R] 3 10.3%
Asn[N] 0 0.0%
Asp[D] 1 3.4%
Cys[C] 0 0.0%
Gln[Q] 0 0.0%
Glu[E] 1 3.4%
Gly[G] 1 3.4%
His[H] 0 0.0%
Ile[I] 1 3.4%
Leu[L] 2 6.9%
Lys[K] 3 10.3%
Met[M] 1 3.4%
Phe[F] 5 17.2%
Pro[P] 1 3.4%
Ser[S] 3 10.3%
Thr[T] 1 3.4%
Trp[W] 0 0.0%
Tyr[Y] 2 6.9%
Val[V] 2 6.9%
Total Length 29

Instability index
Instability index (II) 18.47
This classifies the protein as stable

Atomic composition
Carbon 167
Hydrogen 251
Nitrogen 41
Oxygen 40
Sulfur 1
Total number of atoms 500
Formula = C167H251N41O40S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 67.24

Coding Region Translation Calculations
Codon Bias 0.059
Codon Adaptation Index 0.129
Frequency of Optimal Codons 0.429
Hydropathicity of Protein 0.003
Aromaticity Score 0.241