YAR035C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 0 0.0%
Arg[R] 6 23.1%
Asn[N] 1 3.8%
Asp[D] 0 0.0%
Cys[C] 1 3.8%
Gln[Q] 2 7.7%
Glu[E] 0 0.0%
Gly[G] 0 0.0%
His[H] 0 0.0%
Ile[I] 1 3.8%
Leu[L] 3 11.5%
Lys[K] 1 3.8%
Met[M] 1 3.8%
Phe[F] 1 3.8%
Pro[P] 2 7.7%
Ser[S] 4 15.4%
Thr[T] 0 0.0%
Trp[W] 0 0.0%
Tyr[Y] 3 11.5%
Val[V] 0 0.0%
Total Length 26

Instability index
Instability index (II) 129.41
This classifies the protein as unstable

Atomic composition
Carbon 146
Hydrogen 236
Nitrogen 48
Oxygen 37
Sulfur 2
Total number of atoms 469
Formula = C146H236N48O37S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 4470 M-1 cm-1
assuming NO Cys residues appear as half cystines 4470 M-1 cm-1
assuming all Cys residues are reduced 4470 M-1 cm-1
assuming all pairs of Cys residues form cystines 4470 M-1 cm-1

Aliphatic index
Aliphatic index 60.00

Coding Region Translation Calculations
Codon Bias -0.212
Codon Adaptation Index 0.041
Frequency of Optimal Codons 0.200
Hydropathicity of Protein -1.100
Aromaticity Score 0.154