Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
1 |
0.3% |
| Arg[R] |
8 |
2.5% |
| Asn[N] |
22 |
6.8% |
| Asp[D] |
33 |
10.2% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
20 |
6.2% |
| Glu[E] |
12 |
3.7% |
| Gly[G] |
34 |
10.5% |
| His[H] |
7 |
2.2% |
| Ile[I] |
10 |
3.1% |
| Leu[L] |
32 |
9.9% |
| Lys[K] |
0 |
0.0% |
| Met[M] |
1 |
0.3% |
| Phe[F] |
22 |
6.8% |
| Pro[P] |
1 |
0.3% |
| Ser[S] |
44 |
13.6% |
| Thr[T] |
27 |
8.3% |
| Trp[W] |
16 |
4.9% |
| Tyr[Y] |
0 |
0.0% |
| Val[V] |
34 |
10.5% |
| Total Length |
324 |
|
| Instability index (II) |
30.06 |
| This classifies the protein as stable |
|
| Carbon |
1587 |
| Hydrogen |
2346 |
| Nitrogen |
420 |
| Oxygen |
528 |
| Sulfur |
1 |
| Total number of atoms |
4882 |
| Formula = C1587H2346N420O528S1 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
88000 M-1 cm-1 |
|
|
| Codon Bias |
0.339 |
| Codon Adaptation Index |
0.231 |
| Frequency of Optimal Codons |
0.609 |
| Hydropathicity of Protein |
-0.222 |
| Aromaticity Score |
0.117 |
|
