YHR180C-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 3 8.8%
Arg[R] 4 11.8%
Asn[N] 0 0.0%
Asp[D] 0 0.0%
Cys[C] 2 5.9%
Gln[Q] 3 8.8%
Glu[E] 1 2.9%
Gly[G] 0 0.0%
His[H] 0 0.0%
Ile[I] 3 8.8%
Leu[L] 5 14.7%
Lys[K] 3 8.8%
Met[M] 1 2.9%
Phe[F] 1 2.9%
Pro[P] 2 5.9%
Ser[S] 2 5.9%
Thr[T] 3 8.8%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 1 2.9%
Total Length 34

Instability index
Instability index (II) 85.83
This classifies the protein as unstable

Atomic composition
Carbon 172
Hydrogen 302
Nitrogen 52
Oxygen 45
Sulfur 3
Total number of atoms 574
Formula = C172H302N52O45S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 125 M-1 cm-1
assuming NO Cys residues appear as half cystines 0 M-1 cm-1
assuming all Cys residues are reduced 0 M-1 cm-1
assuming all pairs of Cys residues form cystines 125 M-1 cm-1

Aliphatic index
Aliphatic index 109.12

Coding Region Translation Calculations
Codon Bias 0.126
Codon Adaptation Index 0.128
Frequency of Optimal Codons 0.455
Hydropathicity of Protein 0.035
Aromaticity Score 0.029