YAL016C-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 1 1.6%
Arg[R] 2 3.3%
Asn[N] 5 8.2%
Asp[D] 1 1.6%
Cys[C] 0 0.0%
Gln[Q] 1 1.6%
Glu[E] 5 8.2%
Gly[G] 1 1.6%
His[H] 1 1.6%
Ile[I] 9 14.8%
Leu[L] 6 9.8%
Lys[K] 4 6.6%
Met[M] 1 1.6%
Phe[F] 5 8.2%
Pro[P] 2 3.3%
Ser[S] 5 8.2%
Thr[T] 3 4.9%
Trp[W] 0 0.0%
Tyr[Y] 7 11.5%
Val[V] 2 3.3%
Total Length 61

Instability index
Instability index (II) 47.40
This classifies the protein as unstable

Atomic composition
Carbon 351
Hydrogen 527
Nitrogen 79
Oxygen 95
Sulfur 1
Total number of atoms 1053
Formula = C351H527N79O95S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 10430 M-1 cm-1

Aliphatic index
Aliphatic index 107.05

Coding Region Translation Calculations
Codon Bias 0.242
Codon Adaptation Index 0.243
Frequency of Optimal Codons 0.583
Hydropathicity of Protein 0.013
Aromaticity Score 0.197