YJR005C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 12 12.9%
Arg[R] 11 11.8%
Asn[N] 2 2.2%
Asp[D] 2 2.2%
Cys[C] 0 0.0%
Gln[Q] 9 9.7%
Glu[E] 13 14.0%
Gly[G] 7 7.5%
His[H] 1 1.1%
Ile[I] 1 1.1%
Leu[L] 7 7.5%
Lys[K] 15 16.1%
Met[M] 2 2.2%
Phe[F] 0 0.0%
Pro[P] 1 1.1%
Ser[S] 1 1.1%
Thr[T] 4 4.3%
Trp[W] 1 1.1%
Tyr[Y] 3 3.2%
Val[V] 1 1.1%
Total Length 93

Instability index
Instability index (II) 57.03
This classifies the protein as unstable

Atomic composition
Carbon 463
Hydrogen 779
Nitrogen 155
Oxygen 143
Sulfur 2
Total number of atoms 1542
Formula = C463H779N155O143S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 9970 M-1 cm-1

Aliphatic index
Aliphatic index 49.57

Coding Region Translation Calculations
Codon Bias 0.250
Codon Adaptation Index 0.191
Frequency of Optimal Codons 0.533
Hydropathicity of Protein -1.659
Aromaticity Score 0.043