ADF1/YCL058W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 5 4.4%
Arg[R] 6 5.3%
Asn[N] 3 2.7%
Asp[D] 3 2.7%
Cys[C] 1 0.9%
Gln[Q] 6 5.3%
Glu[E] 12 10.6%
Gly[G] 6 5.3%
His[H] 0 0.0%
Ile[I] 6 5.3%
Leu[L] 9 8.0%
Lys[K] 22 19.5%
Met[M] 5 4.4%
Phe[F] 1 0.9%
Pro[P] 2 1.8%
Ser[S] 11 9.7%
Thr[T] 9 8.0%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 6 5.3%
Total Length 113

Instability index
Instability index (II) 37.23
This classifies the protein as stable

Atomic composition
Carbon 545
Hydrogen 956
Nitrogen 162
Oxygen 173
Sulfur 6
Total number of atoms 1842
Formula = C545H956N162O173S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 0 M-1 cm-1
assuming NO Cys residues appear as half cystines 0 M-1 cm-1
assuming all Cys residues are reduced 0 M-1 cm-1
assuming all pairs of Cys residues form cystines 0 M-1 cm-1

Aliphatic index
Aliphatic index 71.59

Coding Region Translation Calculations
Codon Bias 0.049
Codon Adaptation Index 0.104
Frequency of Optimal Codons 0.444
Hydropathicity of Protein -0.950
Aromaticity Score 0.009