YML007C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 1 2.8%
Arg[R] 5 13.9%
Asn[N] 1 2.8%
Asp[D] 0 0.0%
Cys[C] 0 0.0%
Gln[Q] 1 2.8%
Glu[E] 0 0.0%
Gly[G] 0 0.0%
His[H] 1 2.8%
Ile[I] 4 11.1%
Leu[L] 8 22.2%
Lys[K] 0 0.0%
Met[M] 3 8.3%
Phe[F] 4 11.1%
Pro[P] 1 2.8%
Ser[S] 3 8.3%
Thr[T] 1 2.8%
Trp[W] 0 0.0%
Tyr[Y] 1 2.8%
Val[V] 2 5.6%
Total Length 36

Instability index
Instability index (II) 57.21
This classifies the protein as unstable

Atomic composition
Carbon 208
Hydrogen 339
Nitrogen 55
Oxygen 44
Sulfur 3
Total number of atoms 649
Formula = C208H339N55O44S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 1490 M-1 cm-1

Aliphatic index
Aliphatic index 148.89

Coding Region Translation Calculations
Codon Bias 0.150
Codon Adaptation Index 0.100
Frequency of Optimal Codons 0.455
Hydropathicity of Protein 1.022
Aromaticity Score 0.139