COA3/YJL062W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 6 7.1%
Arg[R] 3 3.5%
Asn[N] 3 3.5%
Asp[D] 5 5.9%
Cys[C] 0 0.0%
Gln[Q] 2 2.4%
Glu[E] 4 4.7%
Gly[G] 4 4.7%
His[H] 2 2.4%
Ile[I] 3 3.5%
Leu[L] 9 10.6%
Lys[K] 10 11.8%
Met[M] 4 4.7%
Phe[F] 4 4.7%
Pro[P] 7 8.2%
Ser[S] 2 2.4%
Thr[T] 6 7.1%
Trp[W] 1 1.2%
Tyr[Y] 6 7.1%
Val[V] 4 4.7%
Total Length 85

Instability index
Instability index (II) 20.90
This classifies the protein as stable

Atomic composition
Carbon 456
Hydrogen 706
Nitrogen 114
Oxygen 123
Sulfur 4
Total number of atoms 1403
Formula = C456H706N114O123S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 14440 M-1 cm-1

Aliphatic index
Aliphatic index 75.76

Coding Region Translation Calculations
Codon Bias 0.064
Codon Adaptation Index 0.106
Frequency of Optimal Codons 0.438
Hydropathicity of Protein -0.484
Aromaticity Score 0.129