YFR012W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 3 10.7%
Arg[R] 2 7.1%
Asn[N] 1 3.6%
Asp[D] 0 0.0%
Cys[C] 0 0.0%
Gln[Q] 3 10.7%
Glu[E] 0 0.0%
Gly[G] 0 0.0%
His[H] 1 3.6%
Ile[I] 0 0.0%
Leu[L] 1 3.6%
Lys[K] 5 17.9%
Met[M] 1 3.6%
Phe[F] 1 3.6%
Pro[P] 4 14.3%
Ser[S] 1 3.6%
Thr[T] 1 3.6%
Trp[W] 0 0.0%
Tyr[Y] 3 10.7%
Val[V] 1 3.6%
Total Length 28

Instability index
Instability index (II) 43.39
This classifies the protein as unstable

Atomic composition
Carbon 155
Hydrogen 243
Nitrogen 45
Oxygen 38
Sulfur 1
Total number of atoms 482
Formula = C155H243N45O38S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 4470 M-1 cm-1

Aliphatic index
Aliphatic index 35.00

Coding Region Translation Calculations
Codon Bias 0.147
Codon Adaptation Index 0.179
Frequency of Optimal Codons 0.481
Hydropathicity of Protein -1.407
Aromaticity Score 0.143