DAD3/YBR233W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 1 1.1%
Arg[R] 3 3.2%
Asn[N] 3 3.2%
Asp[D] 5 5.3%
Cys[C] 0 0.0%
Gln[Q] 12 12.8%
Glu[E] 10 10.6%
Gly[G] 4 4.3%
His[H] 3 3.2%
Ile[I] 5 5.3%
Leu[L] 15 16.0%
Lys[K] 8 8.5%
Met[M] 2 2.1%
Phe[F] 0 0.0%
Pro[P] 2 2.1%
Ser[S] 10 10.6%
Thr[T] 3 3.2%
Trp[W] 0 0.0%
Tyr[Y] 3 3.2%
Val[V] 5 5.3%
Total Length 94

Instability index
Instability index (II) 63.92
This classifies the protein as unstable

Atomic composition
Carbon 471
Hydrogen 776
Nitrogen 132
Oxygen 156
Sulfur 2
Total number of atoms 1537
Formula = C471H776N132O156S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 4470 M-1 cm-1

Aliphatic index
Aliphatic index 99.47

Coding Region Translation Calculations
Codon Bias 0.011
Codon Adaptation Index 0.105
Frequency of Optimal Codons 0.413
Hydropathicity of Protein -0.766
Aromaticity Score 0.032