YGR027W-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 79 4.5%
Arg[R] 71 4.0%
Asn[N] 126 7.2%
Asp[D] 100 5.7%
Cys[C] 15 0.9%
Gln[Q] 78 4.4%
Glu[E] 90 5.1%
Gly[G] 68 3.9%
His[H] 63 3.6%
Ile[I] 128 7.3%
Leu[L] 142 8.1%
Lys[K] 126 7.2%
Met[M] 32 1.8%
Phe[F] 52 3.0%
Pro[P] 109 6.2%
Ser[S] 181 10.3%
Thr[T] 134 7.6%
Trp[W] 14 0.8%
Tyr[Y] 74 4.2%
Val[V] 73 4.2%
Total Length 1755

Instability index
Instability index (II) 48.34
This classifies the protein as unstable

Atomic composition
Carbon 8779
Hydrogen 13786
Nitrogen 2438
Oxygen 2729
Sulfur 47
Total number of atoms 27779
Formula = C8779H13786N2438O2729S47

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 188135 M-1 cm-1
assuming NO Cys residues appear as half cystines 187260 M-1 cm-1
assuming all Cys residues are reduced 187260 M-1 cm-1
assuming all pairs of Cys residues form cystines 188135 M-1 cm-1

Aliphatic index
Aliphatic index 76.56

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000