YFL002W-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 25 5.7%
Arg[R] 14 3.2%
Asn[N] 38 8.7%
Asp[D] 18 4.1%
Cys[C] 3 0.7%
Gln[Q] 33 7.5%
Glu[E] 23 5.3%
Gly[G] 9 2.1%
His[H] 18 4.1%
Ile[I] 22 5.0%
Leu[L] 31 7.1%
Lys[K] 24 5.5%
Met[M] 10 2.3%
Phe[F] 11 2.5%
Pro[P] 32 7.3%
Ser[S] 48 11.0%
Thr[T] 34 7.8%
Trp[W] 4 0.9%
Tyr[Y] 21 4.8%
Val[V] 20 4.6%
Total Length 438

Instability index
Instability index (II) 47.64
This classifies the protein as unstable

Atomic composition
Carbon 2182
Hydrogen 3377
Nitrogen 615
Oxygen 695
Sulfur 13
Total number of atoms 6882
Formula = C2182H3377N615O695S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 53415 M-1 cm-1
assuming NO Cys residues appear as half cystines 53290 M-1 cm-1
assuming all Cys residues are reduced 53290 M-1 cm-1
assuming all pairs of Cys residues form cystines 53415 M-1 cm-1

Aliphatic index
Aliphatic index 66.14

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000