YER159C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 22 5.0%
Arg[R] 14 3.2%
Asn[N] 38 8.6%
Asp[D] 22 5.0%
Cys[C] 3 0.7%
Gln[Q] 28 6.4%
Glu[E] 16 3.6%
Gly[G] 15 3.4%
His[H] 13 3.0%
Ile[I] 23 5.2%
Leu[L] 23 5.2%
Lys[K] 25 5.7%
Met[M] 12 2.7%
Phe[F] 13 3.0%
Pro[P] 42 9.5%
Ser[S] 52 11.8%
Thr[T] 39 8.9%
Trp[W] 3 0.7%
Tyr[Y] 19 4.3%
Val[V] 18 4.1%
Total Length 440

Instability index
Instability index (II) 51.29
This classifies the protein as unstable

Atomic composition
Carbon 2146
Hydrogen 3326
Nitrogen 602
Oxygen 693
Sulfur 15
Total number of atoms 6782
Formula = C2146H3326N602O693S15

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 44935 M-1 cm-1
assuming NO Cys residues appear as half cystines 44810 M-1 cm-1
assuming all Cys residues are reduced 44810 M-1 cm-1
assuming all pairs of Cys residues form cystines 44935 M-1 cm-1

Aliphatic index
Aliphatic index 57.64

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000