YDR365W-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 81 4.6%
Arg[R] 73 4.2%
Asn[N] 128 7.3%
Asp[D] 101 5.8%
Cys[C] 14 0.8%
Gln[Q] 76 4.3%
Glu[E] 92 5.2%
Gly[G] 68 3.9%
His[H] 59 3.4%
Ile[I] 127 7.2%
Leu[L] 141 8.0%
Lys[K] 124 7.1%
Met[M] 32 1.8%
Phe[F] 53 3.0%
Pro[P] 113 6.4%
Ser[S] 178 10.1%
Thr[T] 133 7.6%
Trp[W] 14 0.8%
Tyr[Y] 75 4.3%
Val[V] 73 4.2%
Total Length 1755

Instability index
Instability index (II) 48.53
This classifies the protein as unstable

Atomic composition
Carbon 8783
Hydrogen 13780
Nitrogen 2434
Oxygen 2732
Sulfur 46
Total number of atoms 27775
Formula = C8783H13780N2434O2732S46

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 189625 M-1 cm-1
assuming NO Cys residues appear as half cystines 188750 M-1 cm-1
assuming all Cys residues are reduced 188750 M-1 cm-1
assuming all pairs of Cys residues form cystines 189625 M-1 cm-1

Aliphatic index
Aliphatic index 76.23

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000