YDR261C-D Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 73 4.6%
Arg[R] 67 4.2%
Asn[N] 115 7.2%
Asp[D] 97 6.0%
Cys[C] 13 0.8%
Gln[Q] 72 4.5%
Glu[E] 83 5.2%
Gly[G] 60 3.7%
His[H] 58 3.6%
Ile[I] 113 7.0%
Leu[L] 124 7.7%
Lys[K] 110 6.9%
Met[M] 30 1.9%
Phe[F] 48 3.0%
Pro[P] 107 6.7%
Ser[S] 167 10.4%
Thr[T] 120 7.5%
Trp[W] 13 0.8%
Tyr[Y] 68 4.2%
Val[V] 66 4.1%
Total Length 1604

Instability index
Instability index (II) 50.20
This classifies the protein as unstable

Atomic composition
Carbon 8016
Hydrogen 12543
Nitrogen 2231
Oxygen 2507
Sulfur 43
Total number of atoms 25340
Formula = C8016H12543N2231O2507S43

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 173570 M-1 cm-1
assuming NO Cys residues appear as half cystines 172820 M-1 cm-1
assuming all Cys residues are reduced 172820 M-1 cm-1
assuming all pairs of Cys residues form cystines 173570 M-1 cm-1

Aliphatic index
Aliphatic index 74.11

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000