YPL060C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 29 2.6%
Arg[R] 53 4.8%
Asn[N] 99 9.0%
Asp[D] 71 6.4%
Cys[C] 21 1.9%
Gln[Q] 38 3.4%
Glu[E] 81 7.3%
Gly[G] 42 3.8%
His[H] 30 2.7%
Ile[I] 103 9.3%
Leu[L] 80 7.2%
Lys[K] 106 9.6%
Met[M] 18 1.6%
Phe[F] 33 3.0%
Pro[P] 32 2.9%
Ser[S] 91 8.2%
Thr[T] 82 7.4%
Trp[W] 7 0.6%
Tyr[Y] 39 3.5%
Val[V] 49 4.4%
Total Length 1104

Instability index
Instability index (II) 41.36
This classifies the protein as unstable

Atomic composition
Carbon 5562
Hydrogen 8903
Nitrogen 1573
Oxygen 1758
Sulfur 39
Total number of atoms 17835
Formula = C5562H8903N1573O1758S39

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 97860 M-1 cm-1
assuming NO Cys residues appear as half cystines 96610 M-1 cm-1
assuming all Cys residues are reduced 96610 M-1 cm-1
assuming all pairs of Cys residues form cystines 97860 M-1 cm-1

Aliphatic index
Aliphatic index 80.14

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000