YHL009W-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 53 2.9%
Arg[R] 85 4.7%
Asn[N] 160 8.9%
Asp[D] 123 6.8%
Cys[C] 27 1.5%
Gln[Q] 57 3.2%
Glu[E] 127 7.0%
Gly[G] 67 3.7%
His[H] 49 2.7%
Ile[I] 164 9.1%
Leu[L] 139 7.7%
Lys[K] 182 10.1%
Met[M] 34 1.9%
Phe[F] 51 2.8%
Pro[P] 55 3.1%
Ser[S] 136 7.5%
Thr[T] 121 6.7%
Trp[W] 10 0.6%
Tyr[Y] 71 3.9%
Val[V] 91 5.0%
Total Length 1802

Instability index
Instability index (II) 39.32
This classifies the protein as stable

Atomic composition
Carbon 9140
Hydrogen 14638
Nitrogen 2564
Oxygen 2848
Sulfur 61
Total number of atoms 29251
Formula = C9140H14638N2564O2848S61

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 162415 M-1 cm-1
assuming NO Cys residues appear as half cystines 160790 M-1 cm-1
assuming all Cys residues are reduced 160790 M-1 cm-1
assuming all pairs of Cys residues form cystines 162415 M-1 cm-1

Aliphatic index
Aliphatic index 83.16

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000