YHL009W-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 11 2.7%
Arg[R] 23 5.6%
Asn[N] 35 8.5%
Asp[D] 29 7.0%
Cys[C] 10 2.4%
Gln[Q] 23 5.6%
Glu[E] 30 7.3%
Gly[G] 11 2.7%
His[H] 7 1.7%
Ile[I] 35 8.5%
Leu[L] 39 9.4%
Lys[K] 41 9.9%
Met[M] 7 1.7%
Phe[F] 13 3.1%
Pro[P] 10 2.4%
Ser[S] 22 5.3%
Thr[T] 28 6.8%
Trp[W] 2 0.5%
Tyr[Y] 13 3.1%
Val[V] 24 5.8%
Total Length 413

Instability index
Instability index (II) 36.34
This classifies the protein as stable

Atomic composition
Carbon 2115
Hydrogen 3429
Nitrogen 597
Oxygen 653
Sulfur 17
Total number of atoms 6811
Formula = C2115H3429N597O653S17

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 30995 M-1 cm-1
assuming NO Cys residues appear as half cystines 30370 M-1 cm-1
assuming all Cys residues are reduced 30370 M-1 cm-1
assuming all pairs of Cys residues form cystines 30995 M-1 cm-1

Aliphatic index
Aliphatic index 89.39

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000