YGR109W-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 85 5.5%
Arg[R] 98 6.3%
Asn[N] 89 5.8%
Asp[D] 90 5.8%
Cys[C] 20 1.3%
Gln[Q] 67 4.3%
Glu[E] 90 5.8%
Gly[G] 53 3.4%
His[H] 45 2.9%
Ile[I] 110 7.1%
Leu[L] 142 9.2%
Lys[K] 108 7.0%
Met[M] 29 1.9%
Phe[F] 64 4.1%
Pro[P] 94 6.1%
Ser[S] 91 5.9%
Thr[T] 116 7.5%
Trp[W] 11 0.7%
Tyr[Y] 67 4.3%
Val[V] 78 5.0%
Total Length 1547

Instability index
Instability index (II) 44.88
This classifies the protein as unstable

Atomic composition
Carbon 7982
Hydrogen 12572
Nitrogen 2206
Oxygen 2338
Sulfur 49
Total number of atoms 25147
Formula = C7982H12572N2206O2338S49

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 161580 M-1 cm-1
assuming NO Cys residues appear as half cystines 160330 M-1 cm-1
assuming all Cys residues are reduced 160330 M-1 cm-1
assuming all pairs of Cys residues form cystines 161580 M-1 cm-1

Aliphatic index
Aliphatic index 83.65

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000