YDR034C-D Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 80 4.5%
Arg[R] 74 4.2%
Asn[N] 136 7.7%
Asp[D] 113 6.4%
Cys[C] 15 0.8%
Gln[Q] 84 4.7%
Glu[E] 88 5.0%
Gly[G] 62 3.5%
His[H] 60 3.4%
Ile[I] 130 7.3%
Leu[L] 152 8.6%
Lys[K] 135 7.6%
Met[M] 30 1.7%
Phe[F] 49 2.8%
Pro[P] 97 5.5%
Ser[S] 163 9.2%
Thr[T] 137 7.7%
Trp[W] 15 0.8%
Tyr[Y] 82 4.6%
Val[V] 68 3.8%
Total Length 1770

Instability index
Instability index (II) 46.17
This classifies the protein as unstable

Atomic composition
Carbon 8927
Hydrogen 14026
Nitrogen 2482
Oxygen 2775
Sulfur 45
Total number of atoms 28255
Formula = C8927H14026N2482O2775S45

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 205555 M-1 cm-1
assuming NO Cys residues appear as half cystines 204680 M-1 cm-1
assuming all Cys residues are reduced 204680 M-1 cm-1
assuming all pairs of Cys residues form cystines 205555 M-1 cm-1

Aliphatic index
Aliphatic index 77.80

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000