Q0297 Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 2 3.9%
Arg[R] 6 11.8%
Asn[N] 4 7.8%
Asp[D] 2 3.9%
Cys[C] 0 0.0%
Gln[Q] 0 0.0%
Glu[E] 1 2.0%
Gly[G] 4 7.8%
His[H] 0 0.0%
Ile[I] 1 2.0%
Leu[L] 1 2.0%
Lys[K] 6 11.8%
Met[M] 6 11.8%
Phe[F] 1 2.0%
Pro[P] 8 15.7%
Ser[S] 2 3.9%
Thr[T] 2 3.9%
Trp[W] 0 0.0%
Tyr[Y] 3 5.9%
Val[V] 2 3.9%
Total Length 51

Instability index
Instability index (II) 115.08
This classifies the protein as unstable

Atomic composition
Carbon 257
Hydrogen 419
Nitrogen 79
Oxygen 69
Sulfur 6
Total number of atoms 830
Formula = C257H419N79O69S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours


Aliphatic index
Aliphatic index 30.59

Coding Region Translation Calculations
Codon Bias -0.235
Codon Adaptation Index 0.036
Frequency of Optimal Codons 0.222
Hydropathicity of Protein -1.210
Aromaticity Score 0.078