Q0144 Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 0 0.0%
Arg[R] 0 0.0%
Asn[N] 14 12.8%
Asp[D] 0 0.0%
Cys[C] 1 0.9%
Gln[Q] 1 0.9%
Glu[E] 1 0.9%
Gly[G] 1 0.9%
His[H] 0 0.0%
Ile[I] 18 16.5%
Leu[L] 12 11.0%
Lys[K] 4 3.7%
Met[M] 18 16.5%
Phe[F] 7 6.4%
Pro[P] 1 0.9%
Ser[S] 0 0.0%
Thr[T] 5 4.6%
Trp[W] 1 0.9%
Tyr[Y] 24 22.0%
Val[V] 1 0.9%
Total Length 109

Instability index
Instability index (II) 47.71
This classifies the protein as unstable

Atomic composition
Carbon 685
Hydrogen 989
Nitrogen 129
Oxygen 156
Sulfur 19
Total number of atoms 1978
Formula = C685H989N129O156S19

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 41260 M-1 cm-1
assuming NO Cys residues appear as half cystines 41260 M-1 cm-1
assuming all Cys residues are reduced 41260 M-1 cm-1
assuming all pairs of Cys residues form cystines 41260 M-1 cm-1

Aliphatic index
Aliphatic index 110.00

Coding Region Translation Calculations
Codon Bias -0.616
Codon Adaptation Index 0.082
Frequency of Optimal Codons 0.322
Hydropathicity of Protein 0.715
Aromaticity Score 0.294