Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
3 |
0.8% |
| Arg[R] |
5 |
1.3% |
| Asn[N] |
127 |
31.9% |
| Asp[D] |
10 |
2.5% |
| Cys[C] |
1 |
0.3% |
| Gln[Q] |
4 |
1.0% |
| Glu[E] |
2 |
0.5% |
| Gly[G] |
10 |
2.5% |
| His[H] |
5 |
1.3% |
| Ile[I] |
42 |
10.6% |
| Leu[L] |
41 |
10.3% |
| Lys[K] |
38 |
9.5% |
| Met[M] |
33 |
8.3% |
| Phe[F] |
5 |
1.3% |
| Pro[P] |
7 |
1.8% |
| Ser[S] |
16 |
4.0% |
| Thr[T] |
13 |
3.3% |
| Trp[W] |
3 |
0.8% |
| Tyr[Y] |
28 |
7.0% |
| Val[V] |
5 |
1.3% |
| Total Length |
398 |
|
| Instability index (II) |
41.73 |
| This classifies the protein as unstable |
|
| Carbon |
2051 |
| Hydrogen |
3263 |
| Nitrogen |
595 |
| Oxygen |
611 |
| Sulfur |
34 |
| Total number of atoms |
6554 |
| Formula = C2051H3263N595O611S34 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
58220 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
58220 M-1 cm-1 |
| assuming all Cys residues are reduced |
58220 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
58220 M-1 cm-1 |
|
|
| Codon Bias |
-0.634 |
| Codon Adaptation Index |
0.089 |
| Frequency of Optimal Codons |
0.221 |
| Hydropathicity of Protein |
-0.786 |
| Aromaticity Score |
0.090 |
|
