Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
15 |
2.9% |
| Arg[R] |
13 |
2.5% |
| Asn[N] |
50 |
9.7% |
| Asp[D] |
17 |
3.3% |
| Cys[C] |
6 |
1.2% |
| Gln[Q] |
9 |
1.7% |
| Glu[E] |
12 |
2.3% |
| Gly[G] |
26 |
5.0% |
| His[H] |
10 |
1.9% |
| Ile[I] |
59 |
11.4% |
| Leu[L] |
47 |
9.1% |
| Lys[K] |
46 |
8.9% |
| Met[M] |
33 |
6.4% |
| Phe[F] |
28 |
5.4% |
| Pro[P] |
14 |
2.7% |
| Ser[S] |
32 |
6.2% |
| Thr[T] |
22 |
4.3% |
| Trp[W] |
11 |
2.1% |
| Tyr[Y] |
44 |
8.5% |
| Val[V] |
23 |
4.4% |
| Total Length |
517 |
|
| Instability index (II) |
40.61 |
| This classifies the protein as unstable |
|
| Carbon |
2841 |
| Hydrogen |
4344 |
| Nitrogen |
692 |
| Oxygen |
733 |
| Sulfur |
39 |
| Total number of atoms |
8649 |
| Formula = C2841H4344N692O733S39 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
126435 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
126060 M-1 cm-1 |
| assuming all Cys residues are reduced |
126060 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
126435 M-1 cm-1 |
|
|
| Codon Bias |
-0.310 |
| Codon Adaptation Index |
0.133 |
| Frequency of Optimal Codons |
0.347 |
| Hydropathicity of Protein |
0.010 |
| Aromaticity Score |
0.161 |
|
