AI1/Q0050 Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 31 3.7%
Arg[R] 46 5.5%
Asn[N] 69 8.3%
Asp[D] 34 4.1%
Cys[C] 13 1.6%
Gln[Q] 14 1.7%
Glu[E] 29 3.5%
Gly[G] 51 6.1%
His[H] 17 2.0%
Ile[I] 65 7.8%
Leu[L] 75 9.0%
Lys[K] 83 10.0%
Met[M] 53 6.4%
Phe[F] 30 3.6%
Pro[P] 30 3.6%
Ser[S] 45 5.4%
Thr[T] 61 7.3%
Trp[W] 8 1.0%
Tyr[Y] 39 4.7%
Val[V] 41 4.9%
Total Length 834

Instability index
Instability index (II) 30.81
This classifies the protein as stable

Atomic composition
Carbon 4285
Hydrogen 6890
Nitrogen 1180
Oxygen 1189
Sulfur 66
Total number of atoms 13610
Formula = C4285H6890N1180O1189S66

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 102860 M-1 cm-1
assuming NO Cys residues appear as half cystines 102110 M-1 cm-1
assuming all Cys residues are reduced 102110 M-1 cm-1
assuming all pairs of Cys residues form cystines 102860 M-1 cm-1

Aliphatic index
Aliphatic index 83.44

Coding Region Translation Calculations
Codon Bias -0.162
Codon Adaptation Index 0.123
Frequency of Optimal Codons 0.369
Hydropathicity of Protein -0.334
Aromaticity Score 0.092