Q0032 Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 0 0.0%
Arg[R] 1 1.0%
Asn[N] 10 10.4%
Asp[D] 0 0.0%
Cys[C] 0 0.0%
Gln[Q] 0 0.0%
Glu[E] 0 0.0%
Gly[G] 0 0.0%
His[H] 3 3.1%
Ile[I] 8 8.3%
Leu[L] 12 12.5%
Lys[K] 5 5.2%
Met[M] 16 16.7%
Phe[F] 6 6.2%
Pro[P] 1 1.0%
Ser[S] 5 5.2%
Thr[T] 7 7.3%
Trp[W] 4 4.2%
Tyr[Y] 17 17.7%
Val[V] 1 1.0%
Total Length 96

Instability index
Instability index (II) 42.06
This classifies the protein as unstable

Atomic composition
Carbon 598
Hydrogen 856
Nitrogen 124
Oxygen 136
Sulfur 16
Total number of atoms 1730
Formula = C598H856N124O136S16

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 47330 M-1 cm-1

Aliphatic index
Aliphatic index 84.27

Coding Region Translation Calculations
Codon Bias -0.614
Codon Adaptation Index 0.067
Frequency of Optimal Codons 0.211
Hydropathicity of Protein 0.294
Aromaticity Score 0.281