YLR035C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 47 4.1%
Arg[R] 52 4.5%
Asn[N] 72 6.2%
Asp[D] 70 6.1%
Cys[C] 11 1.0%
Gln[Q] 45 3.9%
Glu[E] 63 5.5%
Gly[G] 48 4.2%
His[H] 40 3.5%
Ile[I] 91 7.9%
Leu[L] 103 8.9%
Lys[K] 96 8.3%
Met[M] 19 1.6%
Phe[F] 33 2.9%
Pro[P] 62 5.4%
Ser[S] 113 9.8%
Thr[T] 84 7.3%
Trp[W] 10 0.9%
Tyr[Y] 51 4.4%
Val[V] 45 3.9%
Total Length 1155

Instability index
Instability index (II) 44.74
This classifies the protein as unstable

Atomic composition
Carbon 5841
Hydrogen 9228
Nitrogen 1614
Oxygen 1787
Sulfur 30
Total number of atoms 18500
Formula = C5841H9228N1614O1787S30

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 131615 M-1 cm-1
assuming NO Cys residues appear as half cystines 130990 M-1 cm-1
assuming all Cys residues are reduced 130990 M-1 cm-1
assuming all pairs of Cys residues form cystines 131615 M-1 cm-1

Aliphatic index
Aliphatic index 80.87

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000