RPL22B/YFL034C-A Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 6 4.9%
Arg[R] 4 3.3%
Asn[N] 6 4.9%
Asp[D] 10 8.2%
Cys[C] 0 0.0%
Gln[Q] 5 4.1%
Glu[E] 8 6.6%
Gly[G] 6 4.9%
His[H] 1 0.8%
Ile[I] 7 5.7%
Leu[L] 8 6.6%
Lys[K] 13 10.7%
Met[M] 1 0.8%
Phe[F] 4 3.3%
Pro[P] 4 3.3%
Ser[S] 10 8.2%
Thr[T] 8 6.6%
Trp[W] 1 0.8%
Tyr[Y] 7 5.7%
Val[V] 13 10.7%
Total Length 122

Instability index
Instability index (II) 34.38
This classifies the protein as stable

Atomic composition
Carbon 619
Hydrogen 977
Nitrogen 161
Oxygen 195
Sulfur 1
Total number of atoms 1953
Formula = C619H977N161O195S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 15930 M-1 cm-1

Aliphatic index
Aliphatic index 83.77

Coding Region Translation Calculations
Codon Bias 0.328
Codon Adaptation Index 0.291
Frequency of Optimal Codons 0.617
Hydropathicity of Protein -0.536
Aromaticity Score 0.098