Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
40 |
13.1% |
| Arg[R] |
11 |
3.6% |
| Asn[N] |
8 |
2.6% |
| Asp[D] |
11 |
3.6% |
| Cys[C] |
8 |
2.6% |
| Gln[Q] |
7 |
2.3% |
| Glu[E] |
11 |
3.6% |
| Gly[G] |
25 |
8.2% |
| His[H] |
9 |
3.0% |
| Ile[I] |
17 |
5.6% |
| Leu[L] |
27 |
8.9% |
| Lys[K] |
9 |
3.0% |
| Met[M] |
11 |
3.6% |
| Phe[F] |
15 |
4.9% |
| Pro[P] |
12 |
3.9% |
| Ser[S] |
24 |
7.9% |
| Thr[T] |
22 |
7.2% |
| Trp[W] |
7 |
2.3% |
| Tyr[Y] |
8 |
2.6% |
| Val[V] |
23 |
7.5% |
| Total Length |
305 |
|
| Instability index (II) |
26.17 |
| This classifies the protein as stable |
|
| Carbon |
1472 |
| Hydrogen |
2281 |
| Nitrogen |
387 |
| Oxygen |
419 |
| Sulfur |
19 |
| Total number of atoms |
4578 |
| Formula = C1472H2281N387O419S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
50920 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
50420 M-1 cm-1 |
| assuming all Cys residues are reduced |
50420 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
50920 M-1 cm-1 |
|
|
| Codon Bias |
0.235 |
| Codon Adaptation Index |
0.168 |
| Frequency of Optimal Codons |
0.530 |
| Hydropathicity of Protein |
0.351 |
| Aromaticity Score |
0.098 |
|
