MLC2/YPR188C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 7 4.3%
Arg[R] 5 3.1%
Asn[N] 8 4.9%
Asp[D] 14 8.6%
Cys[C] 0 0.0%
Gln[Q] 6 3.7%
Glu[E] 21 12.9%
Gly[G] 10 6.1%
His[H] 2 1.2%
Ile[I] 8 4.9%
Leu[L] 19 11.7%
Lys[K] 11 6.7%
Met[M] 4 2.5%
Phe[F] 11 6.7%
Pro[P] 5 3.1%
Ser[S] 11 6.7%
Thr[T] 13 8.0%
Trp[W] 1 0.6%
Tyr[Y] 2 1.2%
Val[V] 5 3.1%
Total Length 163

Instability index
Instability index (II) 36.94
This classifies the protein as stable

Atomic composition
Carbon 817
Hydrogen 1272
Nitrogen 208
Oxygen 274
Sulfur 4
Total number of atoms 2575
Formula = C817H1272N208O274S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 8480 M-1 cm-1

Aliphatic index
Aliphatic index 77.79

Coding Region Translation Calculations
Codon Bias 0.032
Codon Adaptation Index 0.129
Frequency of Optimal Codons 0.430
Hydropathicity of Protein -0.592
Aromaticity Score 0.086