RPO26/YPR187W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 9 5.8%
Arg[R] 9 5.8%
Asn[N] 6 3.9%
Asp[D] 14 9.0%
Cys[C] 0 0.0%
Gln[Q] 6 3.9%
Glu[E] 21 13.5%
Gly[G] 9 5.8%
His[H] 2 1.3%
Ile[I] 9 5.8%
Leu[L] 10 6.5%
Lys[K] 11 7.1%
Met[M] 4 2.6%
Phe[F] 8 5.2%
Pro[P] 8 5.2%
Ser[S] 5 3.2%
Thr[T] 11 7.1%
Trp[W] 1 0.6%
Tyr[Y] 5 3.2%
Val[V] 7 4.5%
Total Length 155

Instability index
Instability index (II) 31.53
This classifies the protein as stable

Atomic composition
Carbon 788
Hydrogen 1222
Nitrogen 210
Oxygen 259
Sulfur 4
Total number of atoms 2483
Formula = C788H1222N210O259S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 12950 M-1 cm-1

Aliphatic index
Aliphatic index 66.71

Coding Region Translation Calculations
Codon Bias 0.208
Codon Adaptation Index 0.244
Frequency of Optimal Codons 0.540
Hydropathicity of Protein -0.875
Aromaticity Score 0.090