Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
62 |
8.1% |
| Arg[R] |
30 |
3.9% |
| Asn[N] |
46 |
6.0% |
| Asp[D] |
46 |
6.0% |
| Cys[C] |
11 |
1.4% |
| Gln[Q] |
37 |
4.8% |
| Glu[E] |
35 |
4.6% |
| Gly[G] |
38 |
4.9% |
| His[H] |
18 |
2.3% |
| Ile[I] |
44 |
5.7% |
| Leu[L] |
79 |
10.3% |
| Lys[K] |
31 |
4.0% |
| Met[M] |
16 |
2.1% |
| Phe[F] |
39 |
5.1% |
| Pro[P] |
48 |
6.2% |
| Ser[S] |
62 |
8.1% |
| Thr[T] |
52 |
6.8% |
| Trp[W] |
6 |
0.8% |
| Tyr[Y] |
28 |
3.6% |
| Val[V] |
40 |
5.2% |
| Total Length |
768 |
|
| Instability index (II) |
41.77 |
| This classifies the protein as unstable |
|
| Carbon |
3818 |
| Hydrogen |
5916 |
| Nitrogen |
1014 |
| Oxygen |
1156 |
| Sulfur |
27 |
| Total number of atoms |
11931 |
| Formula = C3818H5916N1014O1156S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
75345 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
74720 M-1 cm-1 |
| assuming all Cys residues are reduced |
74720 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
75345 M-1 cm-1 |
|
|
| Codon Bias |
0.292 |
| Codon Adaptation Index |
0.229 |
| Frequency of Optimal Codons |
0.574 |
| Hydropathicity of Protein |
-0.211 |
| Aromaticity Score |
0.095 |
|
