KRE6/YPR159W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 42 5.8%
Arg[R] 26 3.6%
Asn[N] 46 6.4%
Asp[D] 59 8.2%
Cys[C] 6 0.8%
Gln[Q] 27 3.8%
Glu[E] 33 4.6%
Gly[G] 55 7.6%
His[H] 15 2.1%
Ile[I] 34 4.7%
Leu[L] 55 7.6%
Lys[K] 20 2.8%
Met[M] 13 1.8%
Phe[F] 33 4.6%
Pro[P] 46 6.4%
Ser[S] 78 10.8%
Thr[T] 50 6.9%
Trp[W] 14 1.9%
Tyr[Y] 41 5.7%
Val[V] 27 3.8%
Total Length 720

Instability index
Instability index (II) 49.82
This classifies the protein as unstable

Atomic composition
Carbon 3558
Hydrogen 5289
Nitrogen 935
Oxygen 1147
Sulfur 19
Total number of atoms 10948
Formula = C3558H5289N935O1147S19

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 138465 M-1 cm-1
assuming NO Cys residues appear as half cystines 138090 M-1 cm-1
assuming all Cys residues are reduced 138090 M-1 cm-1
assuming all pairs of Cys residues form cystines 138465 M-1 cm-1

Aliphatic index
Aliphatic index 64.92

Coding Region Translation Calculations
Codon Bias 0.169
Codon Adaptation Index 0.189
Frequency of Optimal Codons 0.501
Hydropathicity of Protein -0.550
Aromaticity Score 0.122